Trivitron Healthcare's research and development team has developed an RT-PCR-based kit providing fast real-time reports for early detection of Monkeypox virus. (For Research-Use-Only)
overview
Trivitron's Monkeypox Real-Time PCR kit is a multiplex RT-PCR Kit for the rapid qualitative in vitro detection of Monkeypox and Smallpox virus. The assay Kit can distinguish between smallpox and Monkeypox virus.
Features
The kit is based upon Single Step RT-PCR, where both Reverse Transcription by Reverse Transcriptase and PCR by Taq Polymerase takes place in single tube.
Monkey Pox virus kit is developed using four colour fluorescence technology.
A signal from different channel signifies, presence of Orthopox group, Monkeypox & Smallpox virus respectively in the sample.
Assay validation through the additional human internal control gene.
Compatible with both dry swabs as well as sample swabs placed in VTM.
A total turnaround time of 1 hour
Technical Specifications
The kit has been validated on QuantStudio-5/3 and Bio-Rad CFX96, 384 and can also be used on applied Biosystems 7300/7500, Roche Diagnostics LightCycler 96/480, Qiagen Rotor-Gene Q, etc. (Instrument should be calibrated, maintained and used as per manufacturer recommendation).
Sample type: Swab samples collected from surface and/or exudate, roofs from more than one lesion, or lesion crusts.